From the premier cancer center in the world-part 6

From the premier cancer center in the world-part 5

Yadav VR, Prasad S, Gupta SC, Sung B, Phatak SS, Zhang S, Aggarwal BB.
3-Formylchromone interacts with cysteine 38 in p65 protein and with cysteine 179 in I?B? kinase, leading to down-regulation of nuclear factor-?B (NF-?B)-regulated gene products and sensitization of tumor cells.
J Biol Chem. 2012 Jan 2;287(1):245-56.

Fig 2

Prasad S, Yadav VR, Ravindran J, Aggarwal BB.
ROS and CHOP are critical for dibenzylideneacetone to sensitize tumor cells to TRAIL through induction of death receptors and downregulation of cell survival proteins.
Cancer Res. 2011 Jan 15;71(2):538-49.

Fig. 3A

Fig 3D

Fig 4A

Fig. 6B

Gupta SC, Reuter S, Phromnoi K, Park B, Hema PS, Nair M, Aggarwal BB.

Nimbolide sensitizes human colon cancer cells to TRAIL through reactive oxygen species- and ERK-dependent up-regulation of death receptors, p53, and Bax.
J Biol Chem. 2011 Jan 14;286(2):1134-46.

Fig. 4

Phromnoi K, Reuter S, Sung B, Prasad S, Kannappan R, Yadav VR, Chanmahasathien W, Limtrakul P, Aggarwal BB.
A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of I?B kinase activation and sensitizes tumor cells to apoptosis by cytokines and chemotherapeutic agents.
Mol Pharmacol. 2011 Feb;79(2):279-89. Epub 2010 Oct 7.

Fig.1B

Fig 2C

Yadav VR, Prasad S, Kannappan R, Ravindran J, Chaturvedi MM, Vaahtera L, Parkkinen J, Aggarwal BB.
Cyclodextrin-complexed curcumin exhibits anti-inflammatory and antiproliferative activities superior to those of curcumin through higher cellular uptake.
Biochem Pharmacol. 2010 Oct 1;80(7):1021-32.

Fig. 1

Fig. 2

Fig.5

From the premier cancer center in the world-part 5

From the premier cancer center in the world-part 4

From the premier cancer center in the world-part 3

Yadav VR, Prasad S, Aggarwal BB.
Cardamonin sensitizes tumour cells to TRAIL through ROS- and CHOP-mediated up-regulation of death receptors and down-regulation of survival proteins.
Br J Pharmacol. 2012 Feb;165(3):741-53.

Fig. 2

suspiciously blank background (marked by yellow ellipse).
Why is it wrong to “touch up” images? (here)

Fig. 3

red boxed actin loading controls in both experiments are presumably identical despite different experimental conditions.

Usually dividing lines (such as in Fig, 2E) are used to highlight that grouping of images from different parts of the same gel, or from different gels, fields, or exposures has been made (here). Was the purpose of the dividing lines in this case to obscure the identity of the actin lanes?

Fig. 5

Has this circled band been pasted in? Why would you want to do that?

Iyer C, Kosters A, Sethi G, Kunnumakkara AB, Aggarwal BB, Versalovic J.
Probiotic Lactobacillus reuteri promotes TNF-induced apoptosis in human myeloid leukemia-derived cells by modulation of NF-kappaB and MAPK signalling.
Cell Microbiol. 2008 Jul;10(7):1442-52.

Fig. 7

Honest error?

Pandey MK, Sung B, Ahn KS, Kunnumakkara AB, Chaturvedi MM, Aggarwal BB.
Gambogic acid, a novel ligand for transferrin receptor, potentiates TNF-induced apoptosis through modulation of the nuclear factor-kappaB signaling pathway.
Blood. 2007 Nov 15;110(10):3517-25.

Fig 2

Loading controls à la ‘Like Father Like Son?‘: 11 proteins, 1 actin control, how many gels?

Fig 4

Incubation times in fig. 2 and 4 differ but loading controls apparently don’t except that dividing lines were aligned accordingly.

Yadav VR, Prasad S, Reuter S, Sung B, Yamamoto N, Murakami A, Aggarwal BB.
Cardamonin inhibits osteoclastogenesis induced by tumor cells through interruption of the signaling pathway activated by receptor activator of NF-?B ligand.
Cancer Lett. 2011 Dec 17. [Epub ahead of print]

Same images, different experimental conditions?

From the premier cancer center in the world-part 3

The recent posting
From the premier cancer center in the world-part 2
illustrated 10 presumed image manipulations in articles from the lab of Prof. Aggarwal at MD Anderson Cancer Center. Two whistleblowers had contributed images. As it turned out some of the findings were identical to revelations illustrated at this Japanese webwite (Science reported, see here).

From the premier cancer center in the world-part 2

The webpage of MD Anderson Cancer Center says:
“We shall be the premier cancer center in the world, based on the excellence of our people, our research-driven patient care and our science. We are Making Cancer History.”

The Wistar Rat

A whistleblower wrote this story:

Your highly educated readership will know all about the awesome and pioneering contribution to science that is the Wistar Rat Over a century ago, the Wistar Rat was the first strain of laboratory animal to be developed under a systematic breeding program and is still in wide use today. It is all about reproducibility of research results.

Like Father Like Son?

With reference to Never mind the quality, feel the width,

I sent an e-mail to Dr. Gautam Sethi on 24.11.11:
“On the Abnormal Science Blog I have been criticizing the way, you presented some Western blot results using just one actin loading control for up to 19 distinct proteins. Unfortunately, the materials and methods section of the respective paper did not contain sufficient instruction to fully understand the underlying methodology. Since I plan another posting on this topic, the blog readers and the scientific public at large would very much appreciate if you could provide the underlying protocol for this kind of experiment, specifically these informations would be helpful:
how many gels were run, how many blots were performed, how many stripping steps were included.”